The ONE Conference Prague 2013 on April 18th abounded with visionary presentations and fruitful discussions among the more than 150 participants and more than 30 speakers. The ONE project coordinator, Desiree Bua from CSI (IT) and IrinaZalisova from EPMA (CZ) as the Conference Director, officially commenced the proceedings. The following warm-up plenary moderated by Jeremy Millard (DK) was supported by sunny weather; strategies and visions outlined by the keynotes were enriched by discussions at 6 parallel afternoon sessions. Most of presentations are now available on the Conference website. All presented ideas are available in print in the ONE Book of Proceedings, which was handed out to all conference participants, and is also available on-line at the project website.